Gentarget Inc
Gentarget Inc is a premier company that has been serving a global customer base for over 15 years, offering a wide-ranging selection of lentivirus products, totaling over three thousand unique products.
Incorporated in 2008, GenTarget provides proprietary bio-reagents and services with an emphasis on Lentivirus and Adenovirus gene delivery technologies, providing Pre-made Lentiviruses and Adenovirus for gene over-expression, knockdown, miRNA expression, Pre-made mammalian stable cell lines, and more.
With an emphasis on product quality and innovation, we develop and manufacture many proprietary technologies such as the SureTiterTM Lentiviral system; LocLightTMLive cell imaging, LoxP GFP/RFP ColorSwtich for CRE reporting system, and EcoTM instant PCR cloning method, as well as a wide selection of Signal Transduction Pathway Report Lentivirus product series.
Our Mission is to provide easier, better, and more economic solutions that are superior to those currently on the market, for rapidly accelerate drug discovery and bioscience research.
Our Destination is to be a leading provider of lentivirus products and services, and build a long-term developmental relationship with the scientific community through our contracted services.
Our Experts: Our leading scientist has more than 23 years of biomedical and academic research experience, including 18 years of biotech industry experiences, including several major corporations, in vector construction, cloning, protein expression, RNAi screening, bio-arrays, and cDNA library construction and high through-put cloning.
GenTarget Inc. is located in the beautiful Miramar area of San Diego, California, USA. All of its products and services are made within USA. We encourage you to Contact us with your comments, suggestions, and ideas so that we can better serve you. We warmly welcome interested distributors and collaborators to connect with us.
Featured Products
- h AAK1 inducible lentiviral particles – Pre-made inducible lentiviral particles for expressing human target: AAK1 (alternative name: AP2 associated kinase 1), 100% matched NCBI ID: NM_014911.
- AP1-GFP (Bsd) lentivirus – Pre-made lentivirus express GFP reporter under a minimal promoter inserted with 8 tandem repeats of AP1 Responsive Element (AP1-RE), for detecting MAPK / JNK-AP1 signaling pathway acitvity.
- hTERT (CMV, Bsd) lentivirus – Pre-made Lentivirus expressing Human Telomerase Reverse Transcriptase (hTERT, NM_198253.2), under suCMV promoter. A Blasticidin (Bsd) resistant gene was expressed under RSV promoter, which allows to select the transduced cell via Blasticidin antibiotic.
- Anti-BCMA-ScFv-4-1BB-CD3ζ – Pre-made Lentivirus express the CAR construct of “3xFLAG-anti-BCMA-ScFV (VH-VL)-CD8 hinge-4-1BB-CD3ζ”. It does not contain any selection marker, trying to minimize the marker’s possible immunogenicity.
Product Categories
Services
Lentivirus Service
Why use Gentarget’s Lentivirus Services?
- Take advantage of Gentarget’s extensive hand-on experiences in lentivirus system with many proprietary technologies;
- Ues the strongest prompter (suCMV) for the highest protein expression levels in mammalian cells;
- Select most advanced product features for Promoter, Fluorescent tag, Antibiotic selection for high virus titer;
- Fast turnaround time;
- Best quality and best price in the lentivirus fields;
Customized Stable Cell Lines
GenTarget’s stable cell line service provides a very cost-effective way for you to generate a cell line. Using our proprietary lentiviral vectors, we will subclone your target gene into our lentivector, generate the lentivirus particles, sequentially infect the cell line of your interest, and select the cell clones via Cell sorting and antibiotic killing.
For an inducible expression cell line, we will first transduce the cells with TetR repressor lentivirus, to make a TetR stable cell line, then transduce with the target expression lentivirus, to generate the tetracycline-inducible stable cell line.
Our proprietary suCMV promoter has the strongest expression strength in the marker in most cell types, and will provide extremely high levels of expression for your target. Our modified EF1a promoter will express the high level of target in some cell types that has weak CMV promoter strength. Our unique technology ensures a fast turnaround time. We will work with you to ensure that the cell line meets your requirements so that you will get the best quality and the best price.
Fusion In Vivo PCR Cloning
Our proprietary high-throughput Fusion in vivo PCR cloning method eliminates the labor-intensive and sometimes troublesome vector preparation procedures of other cloning techniques. It does not need any kind of In Vitro tube reaction, such as ligation, Topo joining, or In-fusion reaction.
Let E Coli do the job for you in vivo…and do it directly in the expression strain!
With this technology the same PCR product can be used to make both Gateway-compatible Entry and DEST/expression clones without the use of BP or LR enzymes.
GenTarget’s revolutionary Fusion in vivo high throughput cloning technology (patent pending) is an extremely easy PCR cloning method:
- Simply amplify your gene of interest by PCR using a primer pair flanked with short arms homologous to the expression vector.
- Next, add 1µl of your PCR product to the engineered, ready-to-use Cloning Cells and
- Immediately proceed to transform the cells.
- You do not need to buy the expression vectors because the expression-optimized E Coli or mammalian expression vector has already been built into the Cloning Cells. (Note: to use your own vector, we can build it into our engineered cloning cells as a custom service).
For E Coli expression, the verified positive clone will be directly used for T7-based or nonT7-based protein expression (depending on the built-in vector), thus eliminating the second transformation into an expression strain, such as BL21.
Using our Fusion In Vivo technology, We successfully made a large microRNA expression clone collection for one of our clients with a very fast turnaround time and at much lower cost compared to other cloning technologies. We can do the same thing for you! If your collection can be PCRed from genomic DNA or other templates, we can build a collection of shRNA, miRNA or any other.
Protein Expression
E Coli Expression and Purification:
GenTarget’s proprietary Fusion in vivo, PCR cloning technology provides us with an edge when it comes to cloning your target protein into our optimized expression vectors. Our auto-induction medium provides us with a worry-free induction protocol for expression. And in case the expressed protein is very toxic, or expressed mainly in an inclusion body, we will use our self-tight-controlled cloning cells. The expressed protein, with a His tag, will be purified by affinity chromatography. Please contact us for a service quote.
Mammalian Suspension Expression and Purification:
Utilizing GenTarget’s advanced lentiviral vector system, we will clone your target into our lentiviral vector with a His tag, generate expression lentiviral particles, and transduce them into mammalian suspension cells for expression. Under antibiotic selection pressure, only transduced cells will grow and express the target protein. This controlled semi-stable cell line expression system demonstrates high protein expression levels without actually building a stable cell line. The beauty of using the lentiviral system is that lentiviral particles give the highest transduction rate in suspension cells, which can easily generate a large population of target expressing cells. This is a great way to obtain mammalian-expressed protein at a very low cost! The His-tagged expressed proteins will be purified by affinity chromatography. Please contact us for a service quote.
Antibody Cloning and Expression
GenTarget provides antibody engineering and expression services using our own unique optimized protocols. From a hybridoma cell line you provide, we will resolve the variable region and secreted leader sequences of the light- and heavy-chains for isotype determination. We can then insert the variable sequences into our antibody expression vectors in either a one- or two-vector system, and assemble them with the constant regions from human, mouse, or rabbit. The constant regions are codon-optimized for maximum expression in mammalian cells, and the expression leader sequence is engineered for maximum antibody secretion. Upon request, we can also construct any kind of chimeric vector for antibody humanization and engineering. Please Contact us for a quote for this service.
Contact Us
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